Journal: Molecular Therapy

Article Title: Intra-articular Delivery of Antago-miR-483-5p Inhibits Osteoarthritis by Modulating Matrilin 3 and Tissue Inhibitor of Metalloproteinase 2

doi: 10.1016/j.ymthe.2016.12.020

Figure Lengend Snippet: miR-483-5p Directly Targets Matn3 in Chondrocytes during OA Development

Article Snippet: Intra-articular Injection With regard to lentivirus (GenePharma) injection, 10 μL lentivirus-mediated miR-483-5p (5′-AAGACGGGAGAAGAGAAGGGAG-3′) (4 × 10 8 TU/ml), siTimp2 (5′- GGAATGACATCTATGGCAA-3′) (1 × 10 9 TU/mL), or NC (5′- TTCTCCGAACGTGTCACGTTTC-3′), Matn3 ( {"type":"entrez-nucleotide","attrs":{"text":"NM_010770.4","term_id":"306518577","term_text":"NM_010770.4"}} NM_010770.4 ) (Cyagen Biosciences), TIMP2( {"type":"entrez-nucleotide","attrs":{"text":"NM_011594.3","term_id":"61835847","term_text":"NM_011594.3"}} NM_011594.3 ) (Cyagen Biosciences), and corresponding negative controls were injected into the knee joint 34 of male mice (n = 5/group) using a 33G needle and a micro-syringe (Hamilton).

Techniques:

Journal: Oncology Letters

Article Title: Long non-coding RNA DRHC inhibits the proliferation of cancer cells in triple negative breast cancer by downregulating long non-coding RNA HOTAIR

doi: 10.3892/ol.2019.10683

Figure Lengend Snippet: lncRNA DRHC and lncRNA HOTAIR expression levels were dysregulated in triple negative breast cancer tissues. Reverse transcription-quantitative polymerase chain reaction results revealed that (A) lncRNA DRHC was significantly downregulated while (B) lncRNA HOTAIR was significantly upregulated in tumor tissues compared with adjacent healthy tissues. The sample with the lowest expression level was set to ‘1’ and all other samples were normalized to this sample. *P<0.05. lncRNA, long non-coding RNA; DRHC, downregulated in hepatocellular carcinoma; HOTAIR, Hox transcript antisense RNA.

Article Snippet: Cell transfection Vectors expressing lncRNA DRHC ( 11 ) or lncRNA HOTAIR ( {"type":"entrez-nucleotide","attrs":{"text":"NR_047517.1","term_id":"383286742","term_text":"NR_047517.1"}} NR_047517.1 ) as well as the empty pcDNA3.1 vector were purchased from Sangon Biotech Co., Ltd. BT-549 and HCC70 cell lines were cultured overnight to reach 70–80% confluence.

Techniques: Expressing, Reverse Transcription, Real-time Polymerase Chain Reaction

Journal: Oncology Letters

Article Title: Long non-coding RNA DRHC inhibits the proliferation of cancer cells in triple negative breast cancer by downregulating long non-coding RNA HOTAIR

doi: 10.3892/ol.2019.10683

Figure Lengend Snippet: lncRNA DRHC and lncRNA HOTAIR were negatively correlated in TNBC tissues. Pearson's correlation coefficient revealed that the expression levels of lncRNA DRHC and lncRNA HOTAIR were negatively correlated in (A) tumor tissues but not in (B) adjacent healthy tissues. The sample with the lowest expression level was set to ‘1’ and all other samples were normalized to this sample. lncRNA, long non-coding RNA; DRHC, downregulated in HCC; HOTAIR, Hox transcript antisense RNA.

Article Snippet: Cell transfection Vectors expressing lncRNA DRHC ( 11 ) or lncRNA HOTAIR ( {"type":"entrez-nucleotide","attrs":{"text":"NR_047517.1","term_id":"383286742","term_text":"NR_047517.1"}} NR_047517.1 ) as well as the empty pcDNA3.1 vector were purchased from Sangon Biotech Co., Ltd. BT-549 and HCC70 cell lines were cultured overnight to reach 70–80% confluence.

Techniques: Expressing

Journal: Oncology Letters

Article Title: Long non-coding RNA DRHC inhibits the proliferation of cancer cells in triple negative breast cancer by downregulating long non-coding RNA HOTAIR

doi: 10.3892/ol.2019.10683

Figure Lengend Snippet: lncRNA DRHC is a potential upstream inhibitor of lncRNA HOTAIR expression in TNBC cells. Reverse transcription-quantitative polymerase chain reaction results revealed that (A) overexpression of lncRNA DRHC decreased the expression of lncRNA HOTAIR in the TNBC cell lines BT-549 and HCC70 while (B) lncRNA HOTAIR overexpression did not significantly affect the expression level of lncRNA DRH compared with C and NC groups. The sample with the lowest expression level was set to ‘1’ and all other samples were normalized to this sample. DRHC and HOTAIR on the x-axes represent transfection with vectors delivering DRHC and HOTAIR, respectively. *P<0.05. lncRNA, long non-coding RNA; DRHC, downregulated in HCC; HOTAIR, Hox transcript antisense RNA; TNBC, triple negative breast cancer; C, control; NC, negative control.

Article Snippet: Cell transfection Vectors expressing lncRNA DRHC ( 11 ) or lncRNA HOTAIR ( {"type":"entrez-nucleotide","attrs":{"text":"NR_047517.1","term_id":"383286742","term_text":"NR_047517.1"}} NR_047517.1 ) as well as the empty pcDNA3.1 vector were purchased from Sangon Biotech Co., Ltd. BT-549 and HCC70 cell lines were cultured overnight to reach 70–80% confluence.

Techniques: Expressing, Reverse Transcription, Real-time Polymerase Chain Reaction, Over Expression, Transfection, Control, Negative Control

Journal: Oncology Letters

Article Title: Long non-coding RNA DRHC inhibits the proliferation of cancer cells in triple negative breast cancer by downregulating long non-coding RNA HOTAIR

doi: 10.3892/ol.2019.10683

Figure Lengend Snippet: lncRNA DRHC expression level is correlated with tumor size but not tumor metastasis. (A) Expression levels of lncRNA DRHC in tumor tissues decreased significantly as the tumor diameter increased. (B) A significant difference in the expression level of lncRNA DRHC was not observed between the metastasis and non-metastasis groups. The sample with the lowest expression level was set to ‘1’ and all other samples were normalized to this sample. *P<0.05. lncRNA, long non-coding RNA; DRHC, downregulated in HCC; HOTAIR, Hox transcript antisense RNA.

Article Snippet: Cell transfection Vectors expressing lncRNA DRHC ( 11 ) or lncRNA HOTAIR ( {"type":"entrez-nucleotide","attrs":{"text":"NR_047517.1","term_id":"383286742","term_text":"NR_047517.1"}} NR_047517.1 ) as well as the empty pcDNA3.1 vector were purchased from Sangon Biotech Co., Ltd. BT-549 and HCC70 cell lines were cultured overnight to reach 70–80% confluence.

Techniques: Expressing

Journal: Oncology Letters

Article Title: Long non-coding RNA DRHC inhibits the proliferation of cancer cells in triple negative breast cancer by downregulating long non-coding RNA HOTAIR

doi: 10.3892/ol.2019.10683

Figure Lengend Snippet: lncRNA DRHC inhibits the proliferation of TNBC cell lines potentially through lncRNA HOTAIR. The optical density value of the C group at 96 h was set to ‘100’ and all other groups were normalized to this group. Overexpression of lncRNA DRHC inhibited, while overexpression of lncRNA HOTAIR promoted the proliferation of TNBC cell lines. In addition, lncRNA HOTAIR overexpression attenuated the inhibitory effects of lncRNA DRHC overexpression on cancer cell proliferation. *P<0.05. lncRNA, long non-coding RNA; DRHC, downregulated in HCC; TNBC, triple negative breast cancer; HOTAIR, Hox transcript antisense RNA; C, control; NC, negative control.

Article Snippet: Cell transfection Vectors expressing lncRNA DRHC ( 11 ) or lncRNA HOTAIR ( {"type":"entrez-nucleotide","attrs":{"text":"NR_047517.1","term_id":"383286742","term_text":"NR_047517.1"}} NR_047517.1 ) as well as the empty pcDNA3.1 vector were purchased from Sangon Biotech Co., Ltd. BT-549 and HCC70 cell lines were cultured overnight to reach 70–80% confluence.

Techniques: Over Expression, Control, Negative Control